GAD65 antibody (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)) (AA 84-182) Primary Antibody
- Binding Specificity
- AA 84-182
- Human, Mouse, Rat
- This GAD65 antibody is un-conjugated
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Rabbit IgG polyclonal antibody for GAD65 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- E. coli-derived human GAD65 recombinant protein (Position: K84-L182).
- Application Notes
Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Application Details: Western blot, 0.1-0.5&mu,g/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1&mu,g/mL
Direct ELISA, 0.1-0.5&mu,g/mL
- For Research Use only
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C,-20 °C
- Storage Comment
- At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Tian, Ma, Shi, Lou, Liu, Li: "Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, 2015 (PubMed).
- Tian, Ma, Shi, Lou, Liu, Li: "Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, 2015 (PubMed).
- Alternative Name
- GAD2 (GAD2 Antibody Abstract)
Synonyms: Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65
Background: Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.