Patched 1 antibody (C-Term)

Catalog No. ABIN569656

This Goat Polyclonal antibody specifically detects Patched 1 in IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Patched 1 antibody (C-Term) (ABIN569656)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Patched 1 Antibody

Binding Specificity: C-Term

Sequence: ELQDVECEER PR

Specificity: This antibody is expected to recognise all reported isoforms of PTCH1. Variants (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1) encode the same isoform.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of Human PTCH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1

Background: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.Synonyms: PTC, PTC1, PTCH, Patched 1, Protein patched homolog 1

Gene ID: 5727

NCBI Accession: NP_000255

UniProt: Q13635

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation