alpha Adaptin antibody

Catalog No. ABIN5698814

The Rabbit Polyclonal anti-alpha Adaptin antibody has been validated for WB, IHC, ELISA, IP and IF. It is suitable to detect alpha Adaptin in samples from Human, Mouse and Rat.

Quick Overview for alpha Adaptin antibody (ABIN5698814)

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adaptin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-alpha Adaptin Antibody

Immunogen: adaptor-related protein complex 2, alpha 1 subunit

Isotype: IgG

Application Details

Application Notes: WB :1:500-1:5000,IP:1:500-1:5000,IHC:1:20-1:200,IF:1:10-1:100

Comment:

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00323(AP2A1 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for alpha Adaptin

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Alternative Name: alpha Adaptin

Background: Synonyms:ADTAA, alpha Adaptin, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1 Background:This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Molecular Weight: 108kDa

UniProt: O95782

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation