AIF antibody

Catalog No. ABIN5700231

The Rabbit Polyclonal anti-AIF antibody (ABIN5700231) specifically detects AIF in WB, IHC, ELISA, IF and IP. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for AIF antibody (ABIN5700231)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-AIF Antibody

Purpose: AIFM1 antibody

Immunogen: apoptosis-inducing factor, mitochondrion-associated, 1

Isotype: IgG

Application Details

Application Notes: WB :1:1000-1:10000,IP:1:1000-1:10000,IF:1:10-1:100,IHC:1:100-400

Comment:

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab00235(AIF antibody) at dilution of 1:2000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1

Background:

Synonyms: Apoptosis-inducing factor 1, mitochondrial|Programmed cell death protein 8|AIFM1|AIF|PDCD8

Background: This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.

Molecular Weight: 66kDa

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect