PFKM antibody

Catalog No. ABIN5703722

The Rabbit Polyclonal anti-PFKM antibody is suitable to detect PFKM in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for PFKM antibody (ABIN5703722)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-PFKM Antibody

Purpose: PFKM antibody

Immunogen: phosphofructokinase, muscle

Isotype: IgG

Application Details

Application Notes: WB : 1:500-1:5000 IP : 1:500-1:5000 IHC : 1:20-1:200 IF : 1:10-1:100

Comment:

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06341(PFKM antibody) at dilution of 1:800

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM

Background:

Synonyms: ATP-dependent 6-phosphofructokinase, muscle type (ATP-PFK, PFK-M)|6-phosphofructokinase type A|Phosphofructo-1-kinase isozyme A (PFK-A)|Phosphohexokinase|PFKM|PFKX

Background: Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.

Molecular Weight: 75-85 kDa

Gene ID: 5213

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect