Hemoglobin beta F (N-Term) antibody
-
- Target
- Hemoglobin beta F
- Binding Specificity
- N-Term
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- ELISA, Lateral Flow (LF), Western Blotting (WB)
- Characteristics
-
Synonyms: mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Hemoglobin delta subunit, HBG1, HBG2, HbF Antibody
Background: HbF antibodies detect the hemoglobin gamma isoform subunit. Functional alternate hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 gamma subunits (alpha-2 gamma-2). Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. HbF levels are increased to as much as 5 % to 10 % in normal pregnancy. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Hemoglobin variants arise from mutations in the globin genes and sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. HbF antibody does not react other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.
Gene Name: HbG
- Purification
- This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform. Anti-HbF is purified from tissue culture supernatant by protein A purification. This antibody does not react with the HbA, HbS, HbC, or HbA-2 isoforms.
- Immunogen
-
Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.
Immunogen Type: Peptide
- Clone
- 4B3-B5-F3-B7
-
- Application Notes
-
Application Note: Anti-Hemoglobin beta F (MOUSE) antibody is suitable for use in Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:20,000
Suggested Applications: LFA - Comment
-
Matching Control Peptide: ABIN5624580
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
-
-
