ERK1 antibody (N-Term)

Catalog No. ABIN5706741

Supplier Product No.: 600-401-gp0

This anti-ERK1 antibody is a Rabbit Polyclonal antibody detecting ERK1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for ERK1 antibody (N-Term) (ABIN5706741)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ERK1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-gp0

Supplier: Rockland

Purpose: ERK1 N-Term Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human ERK1 protein.

Characteristics: Synonyms: rabbit anti-ERK-1 Antibody, MAPK3, ERK 1, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, microtubule-associated protein 2 kinase, p44-ERK1

Purification: Anti-ERK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-ERK 1 (RABBIT) antibody has been tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43 kDa.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 5.0 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background: Background: ERK1 antibodies detect the ERK1 isoform. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Anti-ERK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins