ACSS2 antibody (N-Term)

Catalog No. ABIN5706867

Product Details anti-ACSS2 Antibody

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics:

Synonyms: rabbit anti-ACSS2, rabbit anti ACSS2, Acetyl-coenzyme A synthetase (cytoplasmic), Acetate-CoA ligase, Acetyl-CoA synthetase, ACS, AceCS, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, ACSS2, ACAS2

Background: This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). ACSS2 (Acyl-CoA synthetase short-chain family member 2) is a protein coding gene encoding a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. This protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. This protein is upregulated in many cancers and is important for pro-survival under hypoxic conditions. ACSS2 may be associated with disorders such as Cystoisosporiasis. Anti-ACSS2 Antibody is useful for researchers interested in parasites, intestines, Glucose Energy Metabolism, and AKT research.

Gene Name: ACSS2

Purification: This affinity purified antibody is directed against human ACSS2 protein. Anti-ACSS2 Antibody to was affinity purified from monospecific antiserum by immunoaffinity chromatography. Blast analysis of the sequence of the immunogen shows 100% identity with human and 80% identity to mouse.

Immunogen:

Immunogen: ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-term of human ACSS2.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-ACSS2 Antibody has been tested in Western Blot and ELISA. Expect a band at approximately 76 kDa in western blot using appropriate lysates. Positive control whole cell lysates used Hep-G2 at 1 μg/mL for WB. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 1:10,000-1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.01 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for ACSS2

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Alternative Name: ACSS2 (ACSS2 Products)

Synonyms: zgc:92200 antibody, wu:fa04c03 antibody, wu:fj80b06 antibody, wu:fj80h04 antibody, ACAS2 antibody, ACECS antibody, ACS antibody, ACSA antibody, dJ1161H23.1 antibody, 1110017C11Rik antibody, ACAS antibody, Acas1 antibody, Acas2 antibody, AceCS1 antibody, Acs1 antibody, acyl-CoA synthetase short chain family member 2 antibody, acetyl-coenzyme A synthetase, cytoplasmic antibody, acyl-CoA synthetase short-chain family member 2 antibody, acyl-CoA synthetase short chain family member 1 antibody, ACSS2 antibody, LOC100179555 antibody, LOC100211402 antibody, acss2 antibody, ACSS1 antibody, Acss2 antibody

Gene ID: 55902

NCBI Accession: NP_001070020

UniProt: Q9NR19