TNF alpha antibody

Catalog No. ABIN5707222

This anti-TNF alpha antibody is a Rat Monoclonal antibody detecting TNF alpha in ELISA, FACS, D and ELISA (Capture). Suitable for Mouse.

Quick Overview for TNF alpha antibody (ABIN5707222)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Detection (D), ELISA (Capture)

Clone: MP6-XT22

Product Details anti-TNF alpha Antibody

Specificity: Mouse/Chicken TNF-α

Characteristics: Rat Anti-Mouse TNF-a-LE/AF

Endotoxin Level: Low endotoxin

Immunogen: E. coli-expressed mouse TNF-alpha

Isotype: IgG1

Application Details

Application Notes:

• Applications: ELISA-Capture - Quality tested , ELISA-Detection - Quality tested , FC - Quality tested , ELISPOT-Capture - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Neut - Reported in literature , Multiplex-Detection - Reported in literature
• Working Dilutions: ELISA Purified (UNLB) antibody ≤ 2 g/mL BIOT conjugate 1:2,000 - 1:4,000 Flow Cytometry FITC conjugate ≤ 1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store at or below -20°C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-a

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome