Thrombomodulin antibody (AA 69-194)

Catalog No. ABIN5707537

Product Details anti-Thrombomodulin Antibody

Target: Thrombomodulin (THBD)

Binding Specificity: AA 69-194

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this recombinant Thrombomodulin antibody.

Clone: RTHBD-1591

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Thrombomodulin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Thrombomodulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: Thrombomodulin / CD141 (THBD Products)

Synonyms: AHUS6 antibody, BDCA3 antibody, CD141 antibody, THPH12 antibody, THRM antibody, TM antibody, AI385582 antibody, THBD antibody, HAST antibody, HAST3 antibody, M-PST antibody, ST1A3/ST1A4 antibody, ST1A5 antibody, STM antibody, TL-PST antibody, thrombomodulin antibody, sulfotransferase family 1A member 3 antibody, THBD antibody, Thbd antibody, SULT1A3 antibody

Background: It recognizes a protein of 75  kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.