DTX2 antibody (Internal Region)

Catalog No. ABIN570807

This anti-DTX2 antibody is a Goat Polyclonal antibody detecting DTX2 in IHC and ELISA. Suitable for Human.

Quick Overview for DTX2 antibody (Internal Region) (ABIN570807)

Target: DTX2 (Deltex Homolog 2 (DTX2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DTX2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-DTX2 Antibody

Binding Specificity: Internal Region

Purpose: DTX2 / Deltext-2

Sequence: EKLSTASGYS DVTD

Specificity: This antibody is expected to recognize both reported isoforms (NP_065943.2, NP_001096066.1). Reported variants NP_001096065.1, NP_065943.2, NP_001096064.1 represent identical protein.

Cross-Reactivity: Cow, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKLSTASGYSDVTD, from the internal region of the protein sequence according to NP_065943.2, NP_001096066.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 85 kDa band in lysates of cell lines A549, HepG2 and NIH3T3 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DTX2

Target: DTX2 (Deltex Homolog 2 (DTX2))

Alternative Name: DTX2

Background: DTX2, deltex homolog 2 (Drosophila), KIAA1528, MGC71098, RNF58, OTTHUMP00000024490, deltex 2, ring finger protein 58, zinc ion binding protein

Gene ID: 113878, 304591

NCBI Accession: NP_065943, NP_001096066

Pathways: Notch Signaling