PDK1 antibody (AA 107-336)

Catalog No. ABIN5708184

The Rabbit Polyclonal anti-PDK1 antibody is suitable to detect PDK1 in samples from Human and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for PDK1 antibody (AA 107-336) (ABIN5708184)

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDK1 Antibody

Binding Specificity: AA 107-336

Purification: Antigen affinity purified

Immunogen: A recombinant human protein corresponding to amino acids V107-Y336 was used as the immunogen for the PDK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PDK1 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL, IHC (FFPE): 1-2 μg/mL, Direct ELISA: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Alternative Name: PDK1 / Pyruvate Dehydrogenase Kinase 1

Background: PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

UniProt: Q15118

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect