CKB antibody

Catalog No. ABIN5708211

The Rabbit Polyclonal anti-CKB antibody is suitable to detect CKB in samples from Human, Rat and Mouse. It has been validated for WB, ELISA and IHC (p).

Quick Overview for CKB antibody (ABIN5708211)

Target: CKB (Creatine Kinase, Brain (CKB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CKB Antibody

Purification: Antigen affinity purified

Immunogen: A recombinant human protein corresponding to amino acids H7-K381 was used as the immunogen for the CKB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CKB antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL, IHC (FFPE): 1-2 μg/mL, Direct ELISA: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CKB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Alternative Name: CKB / Creatine kinase B

Background: Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

UniProt: P12277