NLRP3 antibody (N-Term)

Catalog No. ABIN570839

The Goat Polyclonal anti-NLRP3 antibody has been validated for ELISA and IHC. It is suitable to detect NLRP3 in samples from Human.

Quick Overview for NLRP3 antibody (N-Term) (ABIN570839)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NLRP3 Antibody

Binding Specificity: N-Term

Purpose: Cryopirin / NALP3 / NLRP3

Sequence: RDLYEKAKRD EPK

Specificity: This antibody is expected to recognize all reported isoforms (NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RDLYEKAKRDEPK-C, from the N-Terminus (near) of the protein sequence according to NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human peripheral blood leukocytes. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 36 kDa band in Human Peripheral Blood Mononucleocytes lysates and in lysates of cell line MOLT4, after 0.3-1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the liter

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3

Background: NLRP3, NLR family, pyrin domain containing 3, AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD contain

Gene ID: 114548

NCBI Accession: NP_004886, NP_899632, NP_001120933, NP_001120934

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome