PIAS2 antibody (AA 185-196)

Catalog No. ABIN571134

The Goat Polyclonal anti-PIAS2 antibody (ABIN571134) specifically detects PIAS2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for PIAS2 antibody (AA 185-196) (ABIN571134)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PIAS2 Antibody

Binding Specificity: AA 185-196

Purpose: PIAS2 (aa185-196)

Sequence: PQQVREICIS RD

Specificity: This antibody is expected to recognize both reported isoforms (NP_775298.1, NP_004662.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PQQVREICISRD, from the internal region of the protein sequence according to NP_775298.1, NP_004662.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Spleen and Peripheral Mononucleocytes lysates (calculated MW of 63.4 kDa according to NP_775298.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: PIAS2

Background: PIAS2, protein inhibitor of activated STAT, 2, MGC102682, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, miz, Msx-interacting-zinc finger, protein inhibitor of activated STAT X, zinc finger, MIZ-type containing 4

Gene ID: 9063, 17344, 83422

NCBI Accession: NP_775298, NP_004662

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling