ALDH5A1 antibody (AA 485-496)

Catalog No. ABIN571252

This Goat Polyclonal antibody specifically detects ALDH5A1 in ELISA, WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ALDH5A1 antibody (AA 485-496) (ABIN571252)

Target: ALDH5A1 (Aldehyde Dehydrogenase 5 Family, Member A1 (ALDH5A1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH5A1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ALDH5A1 Antibody

Binding Specificity: AA 485-496

Purpose: ALDH5A1 (aa 485-496)

Sequence: SQDPAQIWRV AE

Specificity: This antibody is expected to recognize both reported isoforms (NP_733936.1, NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SQDPAQIWRVAE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Liver lysates (calculated MW of 58.7 kDa according to NP_733936.1). Recommended concentration: 0.3-1 μg/mL. Please note that both this product and EB10436 detects a 50 kDa in Mouse and Rat Liver lysates (cal

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ALDH5A1

Target: ALDH5A1 (Aldehyde Dehydrogenase 5 Family, Member A1 (ALDH5A1))

Alternative Name: ALDH5A1

Background: ALDH5A1, aldehyde dehydrogenase 5 family, member A1, SSADH, SSDH, NAD(+)-dependent succinic semialdehyde dehydrogenase, aldehyde dehydrogenase 5A1, mitochondrial succinate semialdehyde dehydrogenase, succinate-semialdehyde dehydrogenase

Gene ID: 7915, 214579, 291133

NCBI Accession: NP_733936, NP_001071

Pathways: Monocarboxylic Acid Catabolic Process