GNB3, GNB4 (Internal Region) antibody
Verified
Reactivity: Human
ELISA, WB, IHC
Host: Goat
Polyclonal
unconjugated
1 image
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- Target
- GNB3, GNB4
- Binding Specificity
- Internal Region
- Reactivity
- Human
- Host
- Goat
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Purpose
- GNB3 & GNB4
- Sequence
- SGHDNRVSCL GVT
- Specificity
- This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345, ProteinID: NP_002066.1, NP_067642.1).
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence SGHDNRVSCLGVT, from the internal region of the protein sequence according to NP_002066.1, NP_067642.1.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5 μg/mL.
Western Blot: Approx 35 kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2 kDa according to NP_002066.1. Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- GNB3, GNB4
- Background
- GNB3, guanine nucleotide binding protein (G protein), beta polypeptide 3, G protein, beta-3 subunit, GTP-binding regulatory protein beta-3 chain, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3, guanine nucleotide-binding protein, beta-3
- Gene ID
- 2784, 59345, 60449
- NCBI Accession
- NP_002066, NP_067642
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