ASIC3 antibody (AA 50-100)

Catalog No. ABIN571604

Product Details anti-ASIC3 Antibody

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Binding Specificity: AA 50-100

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASIC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit antibody to extracellular ASIC3 (50-100)

Specificity: Specific for ASIC3.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 50-100 of rat ASIC3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Alternative Name: ASIC3 (ACCN3 Products)

Background: FUNCTION: Cation channel with high affinity for sodium which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4. Interacts with LIN7B MAGI1/BAIAP1 GOPC and ACCN1. SUBCELLULAR LOCATION: Cell membrane, Multi-pass membrane protein. Cytoplasm. Note: Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells. TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain spinal chord lung lymph nodes kidney pituitary heart and testis. DEVELOPMENTAL STAGE: Expressed in fetal tissues expression increases in lung and kidney adult tissues.

UniProt: O35240