Thrombospondin 1 antibody (AA 19-32)

Catalog No. ABIN574081

Product Details anti-Thrombospondin 1 Antibody

Target: Thrombospondin 1 (THBS1)

Binding Specificity: AA 19-32

Reactivity: Human, Cow, Pig, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human THBS1 / Thrombospondin.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bovine, Horse, Pig (100%) Mouse, Rat, Goat, Panda, Rabbit, Opossum (93%) Dog, Bat (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide NRIPESGGDNSVFD from N-Terminus of human THBS1 (NP_003237.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bovine, Horse, Pig (100%), Mouse, Rat, Goat, Panda, Rabbit, Opossum (93%), Dog, Bat (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: THBS1 / Thrombospondin-1 (THBS1 Products)

Synonyms: THBS antibody, THBS-1 antibody, TSP antibody, TSP-1 antibody, TSP1 antibody, Thbs-1 antibody, tbsp1 antibody, thrombospondin-1 antibody, thbs antibody, thbs-1 antibody, tsp antibody, tsp-1 antibody, tsp1 antibody, Tsp1 antibody, THBS1 antibody, Tsp-1 antibody, wu:fc48c03 antibody, si:dkey-11e23.1 antibody, tsp2 antibody, thrombospondin 1 antibody, thrombospondin 1 S homeolog antibody, thrombospondin 1b antibody, thrombospondin 2 L homeolog antibody, THBS1 antibody, Thbs1 antibody, thbs1.S antibody, thbs1b antibody, thbs1 antibody, thbs2.L antibody

Background: Name/Gene ID: THBS1

Synonyms: THBS1, Thrombospondin-1, Thrombospondin-1p180, TSP, TSP1, THBS-1, THBS, Thrombospondin 1, TSP-1

Gene ID: 7057

NCBI Accession: NP_003237

UniProt: P07996

Pathways: Autophagy