HMGB1 antibody (AA 1-216)

Catalog No. ABIN574266

The Mouse Monoclonal anti-HMGB1 antibody (Clone 1D5) (ABIN574266) specifically detects HMGB1 in WB, IHC, ELISA, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for HMGB1 antibody (AA 1-216) (ABIN574266)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1D5

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 1-216

Purification: Purified from ascites by Protein A

Immunogen: HMGB1 (AAH03378, 1 a.a. ~ 216 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IF (10 μg/mL), IHC, IHC-P (5 μg/mL), WB

Usage: Western Blot (Cell lysate) - positive control K-562. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 μg/mL) - positive control HeLa cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMG1 / HMGB1

Background: Name/Gene ID: HMGB1

Synonyms: HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome