SIRT1 antibody (AA 456-555)

Catalog No. ABIN574445

The Mouse Monoclonal anti-SIRT1 antibody is suitable to detect SIRT1 in samples from Human. It has been validated for WB, IHC, ELISA, IF and IHC (p).

Quick Overview for SIRT1 antibody (AA 456-555) (ABIN574445)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7B7

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 456-555

Purification: Purified from ascites by Protein A

Immunogen: SIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IF (10 μg/mL), IHC, IHC-P (5 μg/mL), WB

Usage: Western Blot (Cell lysate) - positive control Hela NE. Immunofluorescence (10 μg/mL) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1 / Sirtuin 1

Background: Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase

Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1

Gene ID: 23411

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling