NF-kB p65 antibody (C-Term)

Catalog No. ABIN576233

The Rabbit Polyclonal anti-NF-kB p65 antibody is suitable to detect NF-kB p65 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IP and EMSA.

Quick Overview for NF-kB p65 antibody (C-Term) (ABIN576233)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA)

Product Details anti-NF-kB p65 Antibody

Binding Specificity: C-Term

Specificity: Recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFkappaB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFkappaB p65 is a 551 amino acid ~65 kD subunit of the NFkappaB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFkappaB appears to be the p65-p50 heterodimer. In the active form NFkappaB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase. NFkappaB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IkappaB In a gel supershift assaywas found to be active against all p65 containing human, mouse or rat NFkappaB complexes using 0.5 μL to 1.0 μL per assay. The binding ofto the NFkappaB p65 subunit can be blocked using the NFkappaB p65 control peptide.

Immunogen: NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), GS, IP, WB (1:2000 - 1:5000)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Serum, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: RELA / NFKB p65

Background: Name/Gene ID: RELA

Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

Gene ID: 5970

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins