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Vimentin antibody (AA 1-466)

This Mouse Monoclonal antibody specifically detects Vimentin in WB, IF, ELISA and ICC. It exhibits reactivity toward Human.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000). (Vimentin antibody (AA 1-466))

ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). (Vimentin antibody (AA 1-466))

2 images

Catalog No. ABIN5775972

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Quick Overview for Vimentin antibody (AA 1-466) (ABIN5775972)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Clone

AT5H8

Discover our top product VIM Primary Antibody

Binding Specificity
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AA 1-466

Purpose

Human Vimentin antibody

Purification

Purified

Immunogen

Recombinant human VIM (1-466aa) purified from E. coli

Isotype

IgG1 kappa
anti-Vimentin (VIM) (AA 371-466) antibody
KO Validated VIM Reactivity: Human WB, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, ELISA, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (C-Term) antibody
VIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS Host: Mouse Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Horse WB, IHC, IF, FACS Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 325-375) antibody
VIM Reactivity: Human, Mouse WB, IHC, ICC, IF (p) Host: Rabbit Monoclonal BLR100G unconjugated Recombinant Antibody

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing cycles

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion, vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.

NCBI Accession

NP_003371

Pathways

Caspase Cascade in Apoptosis