TDG antibody (AA 1-410)

Catalog No. ABIN5776190

The Mouse Monoclonal anti-TDG antibody (Clone AT2F7) (ABIN5776190) specifically detects TDG in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for TDG antibody (AA 1-410) (ABIN5776190)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: AT2F7

Product Details anti-TDG Antibody

Binding Specificity: AA 1-410

Purpose: Human TDG antibody

Purification: Purified

Immunogen: Recombinant human TDG (1-410aa) purified from E. coli

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background: G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. Several bacterial proteins have strong sequence homology with this protein. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Pathways: DNA Damage Repair, Chromatin Binding