Retinoblastoma 1 antibody (Ser780)

Catalog No. ABIN5961792

The Rabbit Polyclonal anti-Retinoblastoma 1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect Retinoblastoma 1 in samples from Human, Mouse and Rat.

Quick Overview for Retinoblastoma 1 antibody (Ser780) (ABIN5961792)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: Ser780

Specificity: Exon 17 tumor GOS561 substitution mutation causes premature stop,GOS563 exon 17 substitution mutation causes premature stop,OSRC,Osteosarcoma,p105-Rb,P105RB,PP105,pp110,PPP1R130,pRb,Prepro retinoblastoma associated protein,Protein phosphatase 1 regulatory subunit 130,Rb,RB transcriptional corepressor 1,RB,RB1,RB1 gene,Retinoblastoma 1,Retinoblastoma suspectibility protein,Retinoblastoma-associated protein

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Rb around the non-phosphorylation site of Ser780.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Molecular Weight: 106kDa

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy