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CHEK1 antibody (pSer317)

The Rabbit Polyclonal anti-CHEK1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect CHEK1 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Horse and Monkey.
Catalog No. ABIN601007

Quick Overview for CHEK1 antibody (pSer317) (ABIN601007)

Target

See all CHEK1 Antibodies
CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity

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Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Monkey

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This CHEK1 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Binding Specificity

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    pSer317

    Specificity

    Recognizes human Chk1 when phosphorylated at serine 317. Species cross-Reactivity: rat.

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic phosphopeptide corresponding to human CHK1 around the phosphorylation site of serine 317 (S-S-SP-Q-P).

    Type of Immunogen: Synthetic phospho-peptide

    Isotype

    IgG
  • Application Notes

    Approved: ELISA (1:4000), IHC (1:50 - 1:100), WB (1:500 - 1:1000)

    Usage: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA:1:4000. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Aliquot to Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
  • Target

    CHEK1 (Checkpoint Kinase 1 (CHEK1))

    Alternative Name

    CHEK1 / CHK1

    Background

    Name/Gene ID: CHEK1
    Subfamily: NIM1
    Family: Protein Kinase

    Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1

    Gene ID

    1111

    UniProt

    O14757

    Pathways

    p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair
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