FOXO1 antibody (pSer256)

Catalog No. ABIN601337

The Rabbit Polyclonal anti-FOXO1 antibody has been validated for WB and IHC. It is suitable to detect FOXO1 in samples from Human, Mouse, Rat, Cow, Pig, Dog, Chicken, Zebrafish (Danio rerio), Monkey, Horse and Xenopus laevis.

Quick Overview for FOXO1 antibody (pSer256) (ABIN601337)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Chicken, Zebrafish (Danio rerio), Monkey, Horse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FOXO1 Antibody

Binding Specificity: pSer256

Specificity: Recognizes human FKHR when phosphorylated at serine 256. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human FKHR around the phosphorylation site of serine 256 (A-A-SP-M-D).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1 / FKHR

Background: Name/Gene ID: FOXO1
Family: Transcription factor

Synonyms: FOXO1, FKHR, Forkhead in rhabdomyosarcoma, FOXO1A, FKH1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead box O1, Forkhead box o1a, Forkhead domain protein fkhr

Gene ID: 2308

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling