H2AFX antibody

Catalog No. ABIN601534

This Rabbit Polyclonal antibody specifically detects H2AFX in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit and Monkey.

Quick Overview for H2AFX antibody (ABIN601534)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-H2AFX Antibody

Specificity: Recognizes human Histone H2AX. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic non-phosphopeptide corresponding to human Histone H2A. X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

Type of Immunogen: Synthetic Non-phosphopeptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:200), IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Immunofluorescence: 1:100-1:200. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AX

Background: Name/Gene ID: H2AFX

Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

Gene ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus