RPS6KB1 antibody (pSer424)

Catalog No. ABIN602253

The Rabbit Polyclonal anti-RPS6KB1 antibody (ABIN602253) specifically detects RPS6KB1 in WB, IHC and IF. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Dog, Pig, Chicken, Monkey, Horse, Goat and Xenopus laevis samples.

Quick Overview for RPS6KB1 antibody (pSer424) (ABIN602253)

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Pig, Chicken, Monkey, Horse, Goat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RPS6KB1 Antibody

Binding Specificity: pSer424

Specificity: Recognizes human RPS6KB1 when phosphorylated at serine 424. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human RPS6KB1 around the phosphorylation site of serine 424 (P-V-SP-P-V).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:200), IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Immunofluorescence:1:100-1:200. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: RPS6KB1 / P70S6K / S6K

Background: Name/Gene ID: RPS6KB1
Subfamily: P70 RSK
Family: Protein Kinase

Synonyms: RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha

Gene ID: 6198

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect