TNF alpha antibody

Catalog No. ABIN602481

The Rat Monoclonal anti-TNF alpha antibody (ABIN602481) specifically detects TNF alpha in IHC, ELISA, FACS, Func and IHC (fro). The antibody is reactive with Mouse and Rat samples.

Quick Overview for TNF alpha antibody (ABIN602481)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TNF alpha Antibody

Specificity: Recognizes mouse TNF, alpha. Species cross-reactivity: rat.

Purification: Protein G purified

Immunogen: Recombinant murine TNF alpha expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1 - 10 μg/mL), Flo (1:50 - 1:100), Func, IHC, IHC-Fr

Usage: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen), and Functional Assays. Flow Cytometry: 1:50-1:100, 10 μL labels 10^6 cells in 100 μL. ELISA: 1-10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives added

Preservative: Without preservative

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome