CDC42 antibody (AA 130-141)

Catalog No. ABIN602960

The Rabbit Polyclonal anti-CDC42 antibody is suitable to detect CDC42 in samples from Human, Mouse, Rat, Cow, Dog, Pig, Monkey, Chicken, Zebrafish (Danio rerio), Rabbit, Horse, Xenopus laevis, Bat and Hamster. It has been validated for WB.

Quick Overview for CDC42 antibody (AA 130-141) (ABIN602960)

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Monkey, Chicken, Zebrafish (Danio rerio), Rabbit, Horse, Xenopus laevis, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC42 Antibody

Binding Specificity: AA 130-141

Specificity: Recognizes human Cdc42, does not cross react with Rac1. Species cross-reactivity: mouse, rat.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Catfish, Salmon, Smelt, Pike, Pufferfish, Zebrafish (100%) Trout (92%) Drosophila, Mosquito (83%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa130-141 of human Cdc42 (AKNKQKPITPET-C). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Catfish, Salmon, Smelt, Pike, Pufferfish, Zebrafish (100%), Trout (92%), Drosophila, Mosquito (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:500 - 1:2000)

Usage: Suitable for use in Western blot: 1:500-1:2000 (0.5-2 μg/mL).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M PBS, 0.15 M sodium chloride,  pH 7.6, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42

Background: Name/Gene ID: CDC42

Synonyms: CDC42, G25K, G25K GTP-binding protein, Growth-regulating protein, CDC42Hs, GTP-binding protein, 25kD

Gene ID: 998

UniProt: P60953

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling