TNF alpha antibody (FITC)

Catalog No. ABIN603914

The Mouse Monoclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Human. It has been validated for WB, FACS and Func.

Quick Overview for TNF alpha antibody (FITC) (ABIN603914)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Product Details anti-TNF alpha Antibody (FITC)

Specificity: Recognizes the free soluble (17kD) and membrane (26kD) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha.

Purification: Purified

Immunogen: Human TNF-alpha.

Type of Immunogen: Protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Func, WB (1:50)

Usage: Suitable for use in Flow Cytometry, Western Blot, and immuno assays. Functional Assays: 1:50. Western Blot: 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Sensitive to light.

Storage: -20 °C

Storage Comment: For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C. Protect from light.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome