GCC2 antibody (AA 1174-1185)

Catalog No. ABIN605021

Product Details anti-GCC2 Antibody

Target: GCC2 (GRIP and Coiled-Coil Domain Containing 2 (GCC2))

Binding Specificity: AA 1174-1185

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GCC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GCC2. This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Horse, Rabbit (92%) Mouse, Bovine, Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKLTNKNNKIED from an internal region of human GCC2 (NP_055450.1, NP_852118.1). Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Horse, Rabbit (92%), Mouse, Bovine, Pig (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 60kD band in Human Lymph Node and Testis lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kD according to NP_055450.1 and of 196kD according to NP_852118.1. The 60kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GCC2

Target: GCC2 (GRIP and Coiled-Coil Domain Containing 2 (GCC2))

Alternative Name: GCC2 (GCC2 Products)

Background: Name/Gene ID: GCC2

Synonyms: GCC2, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC185, GCC protein, 185-kD, Ran-binding protein 2-like 4, RANBP2L4, REN53, KIAA0336

Gene ID: 9648

NCBI Accession: NP_055450, NP_852118

UniProt: Q8IWJ2

Pathways: Maintenance of Protein Location, SARS-CoV-2 Protein Interactome