Bcl-2 antibody (AA 41-54)

Catalog No. ABIN609189

The Mouse Monoclonal anti-Bcl-2 antibody (ABIN609189) specifically detects Bcl-2 in WB, IHC, IF, IP, IHC (p), ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Bcl-2 antibody (AA 41-54) (ABIN609189)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 41-54

Specificity: Recognizes human Bcl-2.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-Fr (0.4 - 1 μg/mL), IHC-P (0.4 - 1 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, and Immunofluorescence. Western Blot: 1-2 μg/mL. Immunohistochemistry (paraffin, frozen): 0.4-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from 1.2 % sodium acetate, 2 mg BSA, 0.01 mg sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2 / Bcl-2

Background: Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling