TNF alpha antibody (FITC)

Catalog No. ABIN610451

The Mouse Monoclonal anti-TNF alpha antibody has been validated for WB, IHC, FACS and Func. It is suitable to detect TNF alpha in samples from Human. There are 2+ publications available.

Quick Overview for TNF alpha antibody (FITC) (ABIN610451)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Functional Studies (Func)

Clone: J1D9

Product Details anti-TNF alpha Antibody (FITC)

Specificity: Human TNF alpha.

Purification: Protein A purified

Immunogen: Recombinant human TNF alpha.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Func, IHC, WB

Usage: Function: Neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 5 % Glycerol, 0.2 % BSA, 0.04 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

References for anti-TNF alpha antibody (FITC) (ABIN610451)

Duchene, Schanstra, Pecher, Pizard, Susini, Esteve, Bascands, Girolami: "A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40375-83, (2002) (PubMed).

Xie, Browning, Hay, Mackman, Ye: "Activation of NF-kappa B by bradykinin through a Galpha(q)- and Gbeta gamma-dependent pathway that involves phosphoinositide 3-kinase and Akt." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24907-14, (2000) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome