AKT1 antibody (C-Term)

Catalog No. ABIN6136733

This anti-AKT1 antibody is a Rabbit Polyclonal antibody detecting AKT1 in WB, IHC, IF and IP. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for AKT1 antibody (C-Term) (ABIN6136733)

Key Features:

• High quality AKT1 Primary Antibody for the detection of AKT1.
• Reliable product with high standard validation data

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-AKT1 Antibody

Binding Specificity: C-Term

Sequence: KEIMQHRFFA GIVWQHVYEK KLSPPFKPQV TSETDTRYFD EEFTAQMITI TPPDQDDSME CVDSERRPHF PQFSYSASGT A

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human AKT1 (NP_005154.2).

Isotype: IgG

Product Specific Information:

What can the AKT1 Antibody ABIN6136733 be used for?: This polyclonal AKT1 Antibody detects AKT1. The AKT1 Antibody has been validated for various applications and can be used for the detection of AKT1 and derivatives by Western Blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.

What validation data is available for this AKT1 Antibody?: The primary antibody is referenced in 3 publications and is characterized by a proven, very high reliability. It has currently 6 product images that show its performance in a variety of applications. The product is currently available in 20 μL, 100 μL, 500 μL, 1000 μL quantities. AKT1 Antibody for the detection of AKT1 and derivatives.

What is the function of AKT1?: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011].

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:100,IF,1:50 - 1:100,IP,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-AKT1 antibody (ABIN6136733)

Li, Yang, Zheng, Xing, Wu, Bian, Wu, Li, Li, Bai, Wu, Jia, Wang, Zhu, Jin: "TNF-α stimulates endothelial palmitic acid transcytosis and promotes insulin resistance." in: Scientific reports, Vol. 7, pp. 44659, (2018) (PubMed).

Oyagbemi, Omobowale, Asenuga, Ochigbo, Adejumobi, Adedapo, Yakubu: "Sodium arsenite-induced cardiovascular and renal dysfunction in rat via oxidative stress and protein kinase B (Akt/PKB) signaling pathway." in: Redox report : communications in free radical research, Vol. 22, Issue 6, pp. 467-477, (2018) (PubMed).

Xie, Liu, Chen, Xu, Zhan, Yang, Li, Zhou: "Umbilical cord-derived mesenchymal stem cells alleviated inflammation and inhibited apoptosis in interstitial cystitis via AKT/mTOR signaling pathway." in: Biochemical and biophysical research communications, Vol. 495, Issue 1, pp. 546-552, (2017) (PubMed).

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.,AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,AKT1,Epigenetics & Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Cancer,Signal Transduction,G protein signaling,Kinase,Serine/threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Protein Kinase C Signaling Pathway,ErbB-HER Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Cell Cycle,G1/S Checkpoint,Cell Adhesion,Microtubules,TGF-b-Smad Signaling Pathway,ESC Pluripotency and Differentiation,Endocrine & Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Warburg Effect,Immunology & Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,Cardiovascular,Angiogenesis,AKT1

Molecular Weight: 48 kDa/55 kDa

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect