EAF2 antibody (AA 1-170)

Catalog No. ABIN6139905

Product Details anti-EAF2 Antibody

Target: EAF2 (ELL Associated Factor 2 (EAF2))

Binding Specificity: AA 1-170

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Sequence: MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for EAF2

Target: EAF2 (ELL Associated Factor 2 (EAF2))

Alternative Name: EAF2 (EAF2 Products)

Synonyms: eaf2 antibody, MGC69052 antibody, MGC68453 antibody, zgc:86600 antibody, wu:fb76c01 antibody, wu:fc43g12 antibody, BM040 antibody, TRAITS antibody, U19 antibody, AW048865 antibody, FESTA-L antibody, FESTA-S antibody, Festa antibody, Traits antibody, ELL associated factor 2 S homeolog antibody, ELL associated factor 2 L homeolog antibody, ELL associated factor 2 antibody, eaf2.S antibody, eaf2.L antibody, eaf2 antibody, EAF2 antibody, Eaf2 antibody

Background: Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF2,BM040,TRAITS,U19,Epigenetics & Nuclear Signaling,Cancer,Tumor suppressors,Cell Biology & Developmental Biology,Apoptosis,EAF2

Molecular Weight: 14 kDa/28 kDa

Gene ID: 55840

UniProt: Q96CJ1