POLG antibody (AA 829-1201)

Catalog No. ABIN615477

The Rabbit Polyclonal anti-POLG antibody (ABIN615477) specifically detects POLG in WB, IHC (p) and IF. The antibody is reactive with Human samples.

Quick Overview for POLG antibody (AA 829-1201) (ABIN615477)

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-POLG Antibody

Binding Specificity: AA 829-1201

Specificity: This antibody detects POLG1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.7 mg/mL

Buffer: PBS, pH 7, 1 % BSA, 20 % Glycerol and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Dilute only prior to immediate use.

Target Details for POLG

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Alternative Name: POLG1

Background: As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil DNA glycosylase and apurinic / apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic / apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.Synonyms: DNA polymerase subunit gamma-1, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLGA, PolG-alpha

Gene ID: 5428

NCBI Accession: NP_001119603

UniProt: P54098