SELPLG antibody (CF®594)
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- Target See all SELPLG Antibodies
- SELPLG (Selectin P Ligand (SELPLG))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SELPLG antibody is conjugated to CF®594
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt)
- Purpose
- Mouse Monoclonal anti-CD162 (PSGL1/1601), CF594 Conjugate
- Characteristics
- CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human full-length SELPLG (CD162) protein
- Clone
- PSGL1-1601
- Isotype
- IgG1
- Top Product
- Discover our top product SELPLG Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Jurkat cells. Tonsil or Spleen
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- SELPLG (Selectin P Ligand (SELPLG))
- Alternative Name
- CD162 (SELPLG Products)
- Synonyms
- CD162 antibody, CLA antibody, PSGL-1 antibody, PSGL1 antibody, SELPLG antibody, SELPG antibody, Psgl-1 antibody, Psgl1 antibody, Selp1 antibody, Selpl antibody, selectin P ligand antibody, selectin, platelet (p-selectin) ligand antibody, clasper antibody, SELPLG antibody, Selplg antibody, cla antibody
- Background
- CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein lig, 1, PSGL1, Selectin P lig, SELPLG
- Molecular Weight
- 43-120 kDa
- Gene ID
- 6404, 591014
- UniProt
- Q14242
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