CD47 antibody (CF®647)

Catalog No. ABIN6159731

This anti-CD47 antibody is a Mouse Monoclonal antibody detecting CD47 in FACS, IF and IHC (f). Suitable for Human and Mouse.

Quick Overview for CD47 antibody (CF®647) (ABIN6159731)

Target: CD47

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: IAP-964 B6H12-2

Product Details anti-CD47 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD47 (IAP/964 + B6H12.2), CF647 Conjugate

Characteristics: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human IAP protein (IAP/964), Intact CD47 purified from placenta (B6H12.2)

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen

Molecular Weight: ~50 kDa

Gene ID: 961, 446414

UniProt: Q08722