Desmoglein 1 antibody (Biotin)

Catalog No. ABIN6162146

Product Details anti-Desmoglein 1 Antibody (Biotin)

Target: Desmoglein 1 (DSG1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 1 antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-Desmoglein-1 (DSG1/1733), Biotin Conjugate

Characteristics: This antibody recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain (exact sequence is proprietary)

Clone: DSG1-1733

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA  pH 8.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431 or A-375 cells. Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: Desmoglein-1 (DSG1 Products)

Synonyms: CDHF4 antibody, DG1 antibody, DSG antibody, PPKS1 antibody, SPPK1 antibody, DSG-1 antibody, DSG1 antibody, Dsg1b antibody, Dsg1c antibody, desmoglein 1 antibody, desmoglein 4 antibody, DSG1 antibody, DSG4 antibody, Dsg1 antibody

Background: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1

Molecular Weight: 150 kDa (precursor), 160 kDa (mature)

Gene ID: 1828, 2633

UniProt: Q02413

Pathways: Cell-Cell Junction Organization