NAPSA antibody (CF®647)

Catalog No. ABIN6165963

Product Details anti-NAPSA Antibody (CF®647)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPSA antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Napsin-A (NAPSA/1238), CF647 Conjugate

Characteristics: Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Napsin-A protein

Clone: NAPSA-1238

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 1-2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Lung adenocarcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: Napsin-A (NAPSA Products)

Synonyms: KAP antibody, Kdap antibody, NAP1 antibody, NAPA antibody, SNAPA antibody, pronapsin antibody, kap antibody, kdap antibody, nap1 antibody, napa antibody, napsa antibody, snapa antibody, napsin A aspartic peptidase antibody, napsin A aspartic peptidase L homeolog antibody, NAPSA antibody, Napsa antibody, napsa.L antibody

Background: ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Molecular Weight: 37 kDa

Gene ID: 9476, 512843

UniProt: O96009

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis