Transgelin antibody (CF®594)

Catalog No. ABIN6167728

Product Details anti-Transgelin Antibody (CF®594)

Target: Transgelin (TAGLN)

Reactivity: Human, Mouse, Cow, Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transgelin antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-Transgelin / SM22-alpha (TAGLN/247), CF594 Conjugate

Characteristics: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Clone: TAGLN-247

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mLImmunofluorescence 1-2 μg/mL

Comment:

U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: Transgelin / SM22-alpha (TAGLN Products)

Synonyms: MGC68550 antibody, sm22 antibody, smcc antibody, tagln1 antibody, ws3-10 antibody, MGC107855 antibody, SM22 antibody, SMCC antibody, TAGLN1 antibody, WS3-10 antibody, Sm22 antibody, Sm22a antibody, Ws310 antibody, SM22A antibody, zgc:153186 antibody, transgelin L homeolog antibody, transgelin antibody, tagln.L antibody, tagln antibody, LOC732906 antibody, TAGLN antibody, tagl antibody, LOC100009332 antibody, Tagln antibody

Molecular Weight: 22 kDa

Gene ID: 6876, 410977

UniProt: Q01995