Myogenin antibody (AA 30-224) (CF®594)

Catalog No. ABIN6171055

Product Details anti-Myogenin Antibody (CF®594)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Binding Specificity: AA 30-224

Reactivity: Human, Mouse, Rat, Pig, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is conjugated to CF®594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-Myogenin (MGN185 + F5D), CF594 Conjugate

Characteristics: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms? tumor. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human myogenin recombinant protein (MGN185), Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)

Clone: MGN185 F5D

Isotype: IgG

Application Details

Application Notes: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin (MYOG Products)

Synonyms: cb553 antibody, zgc:100763 antibody, LOC446037 antibody, myf4 antibody, Xmyog antibody, myogenin antibody, LOC100136471 antibody, LOC100224062 antibody, LOC100303673 antibody, MYF4 antibody, bHLHc3 antibody, myo antibody, myf-4 antibody, myogenin antibody, myogenin (myogenic factor 4) antibody, MYOG antibody, myog antibody, LOC100303673 antibody, Myog antibody

Background: BHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Molecular Weight: 34 kDa

Gene ID: 4656, 2830

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development