CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (CF®647)

Catalog No. ABIN6174306

Product Details anti-CDKN1B Antibody

Target: CDKN1B

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDKN1B antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)

Purpose: Mouse Monoclonal anti-p27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8)), CF647 Conjugate

Characteristics: This antibody recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Mouse recombinant p27 protein (DCS-72.F6), Recombinant human CDKN1B protein (KIP1/769)

Clone: DCS-72-F6 KIP1-769

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CDKN1B

Target: CDKN1B

Alternative Name: p27 / KIP1 (CDKN1B Products)

Synonyms: CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B, Kip1, p27, FAM14D, ISG12, ISG12A, P27, Cdki1b, AA408329, AI843786, p27Kip1, cdkn1bl, cyclin dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1Bb, interferon alpha inducible protein 27, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1Ba, CDKN1B, cdkn1bb, IFI27, Cdkn1b, cdkn1ba

Background: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Molecular Weight: 25-26 kDa

Gene ID: 1027, 238990

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy