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CDKN1B antibody (CF®647)

CDKN1B Reactivity: Human, Mouse, Rat, Monkey WB, IF, FACS, IHC (f) Host: Mouse Monoclonal DCS-72-F6 KIP1-769 CF®647
Catalog No. ABIN6174306
  • Target See all CDKN1B Antibodies
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Reactivity
    • 244
    • 113
    • 101
    • 25
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Monkey
    Host
    • 211
    • 46
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 204
    • 57
    Monoclonal
    Conjugate
    • 134
    • 18
    • 16
    • 13
    • 13
    • 11
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This CDKN1B antibody is conjugated to CF®647
    Application
    • 181
    • 116
    • 62
    • 59
    • 46
    • 41
    • 33
    • 25
    • 22
    • 16
    • 16
    • 11
    • 7
    • 7
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-p27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8)), CF647 Conjugate
    Characteristics
    This antibody recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Mouse recombinant p27 protein (DCS-72.F6), Recombinant human CDKN1B protein (KIP1/769)
    Clone
    DCS-72-F6 KIP1-769
    Isotype
    IgG
    Top Product
    Discover our top product CDKN1B Primary Antibody
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Alternative Name
    p27 / KIP1 (CDKN1B Products)
    Background
    CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
    Molecular Weight
    25-26 kDa
    Gene ID
    1027, 238990
    UniProt
    P46527
    Pathways
    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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