CD22 antibody (CF®640R)

Catalog No. ABIN6181978

The Mouse Monoclonal anti-CD22 antibody has been validated for FACS and IF. It is suitable to detect CD22 in samples from Human, Mouse and Rat.

Quick Overview for CD22 antibody (CF®640R) (ABIN6181978)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is conjugated to CF®640R

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: Myg13

Product Details anti-CD22 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-CD22 (Myg13), CF640R Conjugate

Characteristics: This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human Raji cells

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human tonsil and lymphocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Molecular Weight: 130-140 kDa

Gene ID: 933, 579691

UniProt: P20273