CD22 antibody (AA 52-178) (CF®568)

Catalog No. ABIN6182026

The Mouse Monoclonal anti-CD22 antibody has been validated for FACS, WB, ELISA, IHC, IF and Coat. It is suitable to detect CD22 in samples from Human.

Quick Overview for CD22 antibody (AA 52-178) (CF®568) (ABIN6182026)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat)

Clone: BLCAM-1795

Product Details anti-CD22 Antibody (CF®568)

Binding Specificity: AA 52-178

Purpose: Mouse Monoclonal anti-CD22 / BL-CAM (BLCAM/1795), CF568 Conjugate

Characteristics: This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BL-CAM/CD22 protein (aa52-178) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22 / BL-CAM

Background: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14

Molecular Weight: 130-140 kDa

Gene ID: 933, 579691

UniProt: P20273