Recombinant CD15 antibody (Biotin)

Catalog No. ABIN6183865

The Rabbit Monoclonal anti-CD15 antibody has been validated for FACS, IHC and IF. It is suitable to detect CD15 in samples from Human, Mouse and Rat.

Quick Overview for Recombinant CD15 antibody (Biotin) (ABIN6183865)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD15 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: FUT4-1478R

Product Details anti-CD15 Antibody (Biotin)

Purpose: Recombinant Rabbit Monoclonal anti-CD15 / FUT4 (FUT4/1478R), Biotin Conjugate

Characteristics: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human neutrophils

Isotype: IgG kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Flow cytometry 2-4 μg/million cells
• Immunofluorescence 2-4 μg/mL
• Immunohistology (formalin) 5-10 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

U937 cells, Reed-Sternberg',s cells in Hodgkin',s lymphoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15 / FUT4

Molecular Weight: 220 kDa

Gene ID: 2526, 654379

UniProt: P22083