CD46 antibody (CF®647)

Catalog No. ABIN6187872

This Mouse Monoclonal antibody specifically detects CD46 in FACS and IF. It exhibits reactivity toward Human and Baboon.

Quick Overview for CD46 antibody (CF®647) (ABIN6187872)

Target: CD46

Reactivity: Human, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1-E4-3

Product Details anti-CD46 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD46 (169-1-E4.3), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 52 kDa-58 kDa, identified as CD46 (also known as membrane cofactor protein, MCP). Its epitope is located on the four immunogenic short consensus repeat (SCR) domains at N-terminus of the protein. CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Does not react with horse, others not tested

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

K562, U937, HL60, HeLa and Jurkat cells, and human lymphocytes. Lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD46

Target: CD46

Alternative Name: CD46

Background: AHUS2, Complement membrane cofactor protein, MCP, Measles virus receptor, Membrane cofactor protein, MIC10, TLX, Trophoblast leucocyte common antigen, Trophoblast lymphocyte cross reactive antigen

Molecular Weight: 52-58 kDa

Gene ID: 4179, 510402

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization