PAX7 antibody (AA 300-600)

Catalog No. ABIN6189191

Product Details anti-PAX7 Antibody

Target: PAX7 (Paired Box 7 (PAX7))

Binding Specificity: AA 300-600

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Mouse Monoclonal anti-PAX7 (PAX7/497), Purified, with BSA

Characteristics: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant fragment (aa300-600) of human PAX7 protein

Clone: PAX7-497

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, Jurkat, A431, MCF-7, U2OS or NIH/3T3 cells. Rhabdomyosarcoma (RMS)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7 (PAX7 Products)

Synonyms: PAX7 antibody, HUP1 antibody, PAX7B antibody, RMS2 antibody, pax-7 antibody, PAX-7 antibody, RGD1564360 antibody, Pax-7 antibody, pax7 antibody, pax7c antibody, pax7d antibody, pax7e antibody, paired box 7 antibody, paired box protein Pax-7 antibody, paired box 7 L homeolog antibody, paired box 7a antibody, PAX7 antibody, pax7.L antibody, LOC536229 antibody, Pax7 antibody, pax7a antibody

Background: HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

Molecular Weight: 57 kDa

Gene ID: 5081, 113253

UniProt: P23759